The Effect of Sambiloto (Andrographis paniculata Nees.) and Kejibeling (Strobilanthes crispa) Leaves on Uric Acid Levels of White Rats (Rattus norvegicus)

Abstract

This study aims to seek answers to four research questions, (1) Do the extracts of Sambiloto (Andrographis paniculata Nees.) and Kejibeling (Strobilanthes crispa) leaves affect the blood uric acid levels of white rats (Rattus norvegicus)? (2) Do different dose concentrations of Sambiloto (Andrographis paniculata Nees.) and Kejibeling (Strobilanthes crispa) leaves extracts affect the blood uric acid level of white rats (Rattus norvegicus)? (3) And do time exposures of Sambiloto (Andrographis paniculata Nees.) and Kejibeling (Strobilanthes crispa) leaves extracts affect the blood uric acid level of white rats (Rattus norvegicus)? (4) Do the interactions of the variables in this study affect the blood uric acid of white rats (Rattus novergicus)? This laboratory experimental research design used twenty-eight white rats, divided into two negative and positive control groups and six treatment groups. Rats were acclimatized for fourteen days and were fasted before the data collection. The data are blood uric acid levels of the blood samples taken from the cut made at the tip of the tail. The variables of the study are the extracts (two) of the leaves, the different levels (three) of dosages of the extract, and the length times (four) exposures of the animals after being given the extract orally. Univariate Statistical Analysis was used to analyze the data. The results of the analysis show that there are no statistically significant differences in the effect of the different kinds of leaves extracts (p = 0.994), the different dosages (p = 0.574), the length of times of exposure (p = 0,112), and also in the interactions of the variables on the levels of the uric acid, Dose*Time with p = 0.895, Dose*Group with p = 0.266, Time*Group with p = 0.511, but in the interaction of Dose*Time*Group is significant with p = 0.047. However, on the descriptive study, the results show that the leaves extracts, the different dose concentrations used, and the length of times of exposure show that the uric acid levels were lowered. Thus, this study concluded that Sambiloto (Andrographis paniculata Nees.) and Kejibeling (Strobilanthes crispa) leaves extracts to lower the blood uric acid levels of white rats (Rattus novergicus).

Keywords: Sambiloto, Kejibeling, Uric Acid, White Rats.